Center for Environmental Genetics Career Development Program              

The CEG Career Development Program has two distinct but related tracks to support the development of the next generation of environmental health sciences scientists. These are basic, translational, or clinical investigators who have the passion, optimism, and technological skills to conduct transdisciplinary research to advance EHS research with avant-garde discoveries. The first pathway is the Next-Generation Biomedical Investigator (NGBI), designed to assist junior faculty in their transition to environmental health science researchers.

The second pathway is the New Investigator Scholar award, designed to assist clinicians and health practitioners learn environmental health science concepts, and bring these concepts into translational research practice. University of Cincinnati or Cincinnati Children's Hospital Medical Center faculty who have an interest in Environmental Health Sciences research are encouraged to contact the CEG office for more information.  Support is provided to students for dissertation research expenses, career development, travel to professional meetings, and other activities.

Contacts and most recent requests for applications are on the main Career Development Program page.

Next Generation Biomedical Investigators – Awards and Progress:

2007-08 Tianying Wu, MD, PhD, ScM

Dr. Wu was our very first CEG NGBI.  The CEG provided her with salary support, research supplies, and mentorship to develop a research program. Dr. Wu has long-standing interests in modification of diet to improve health through nutrition interventions and the implication of nutrition epidemiology methods in environmental epidemiology, focusing on the interaction between nutrition and occupational exposures in the development of complex diseases.

Grants:

  • Exposure-Induced Systemic Oxidative Stress in Asthmatic Children  Awarded 7/09-6/11  CCHMC
  • 2008-09  UC Barrett Cancer Center Pilot Grant ($20,000)
  • 2009-10  UC Medical Center Dean Bridge Funds ($60,000)
  • 2009-11  Co-investigator R21 ES016830-01A1(Kovacic) DEP Exposure Aggravates Atopy in Gene-Predisposed Children
  • 2009-13 Collaborator K23 DK083528 Patient Oriented Career Development Award (Lee) Oxidative stress and arteriovenous fistula maturation
  • 2009-13 Collaborator (Eliassen) R01CA131218 Oxidative stress, antioxidants and risk of breast cancer: a prospective analysis
  • 2011 CEG Director’s Discretionary Funds ($25,000)
  • Global and Specific Oxidation Pathways Related to Prostate Cancer, Prospective analysis.  Awarded. 9/8/2010-8/31/2015 (1K07CA138714-01A2) 

Publications:

Wu T, Giovannucci E, Welge J, Mallick P, Tang WY, Ho SM. Measurement of GSTP1 promoter methylation in body fluids may complement PSA screening: a meta-analysis. Br J Cancer. 2011 Jun 28;105(1):65-73. doi: 10.1038/bjc.2011.143.  PubMed PMID: 21654682.  Read the general public discussion of this research: http://healthnews.uc.edu/news/?/14244/
See the news video!

2008 – 2009

Ge Zhang, PhD—

  • Awarded $31,000 from Dean’s bridge funding program for his proposal, “An Integrative Informatics Platform for Community-Based Translational Genomic Research” aimed to develop an integrative informatics platform to integrate genomic and clinical information and enhance collaboration and communication between varying parties involved in translational genomic research.
  • 2010 promoted to Assistant Professor at CCHMC
  • Publications (CEG members in bold, CEG trainees in bold/italic)
  • Karns R, Zhang G, Sun G, Rao Indugula S, Cheng H, Havas-Augustin D, Novokmet N, Rudan D, Durakovic Z, Missoni S, Chakraborty R, Rudan P, Deka R. Genome-wide association of serum uric Acid concentration: replication of sequence variants in an island population of the Adriatic coast of croatia. Ann Hum Genet. 2012
    Mar;76(2):121-7. doi: 10.1111/j.1469-1809.2011.00698.x. Epub 2012 Jan 9. PubMed PMID: 22229870.

    Zhang G, Karns R, Sun G, Indugula SR, Cheng H, Havas-Augustin D, Novokmet N, Rudan D, Durakovic Z, Missoni S, Chakraborty R, Rudan P, Deka R. Extent of height variability explained by known height-associated genetic variants in an isolated population of the Adriatic coast of Croatia. PLoS One. 2011;6(12):e29475. Epub 2011 Dec 27. PubMed PMID: 22216288; PubMed Central PMCID: PMC3246488.

    Deka R, Durakovic Z, Niu W, Zhang G, Karns R, Smolej-Narancic N, Missoni S, Caric D, Caric T, Rudan D, Salzer B, Chakraborty R, Rudan P. Prevalence of metabolic syndrome and related metabolic traits in an island population of the Adriatic. Ann Hum Biol. 2012 Jan;39(1):46-53. Epub 2011 Dec 12. PubMed PMID:22149059.

    Karns R, Zhang G, Jeran N, Havas-Augustin D, Missoni S, Niu W, Indugula SR, Sun G, Durakovic Z, Narancic NS, Rudan P, Chakraborty R, Deka R. Replication of genetic variants from genome-wide association studies with metabolic traits in an island population of the Adriatic coast of Croatia. Eur J Hum Genet. 2011 Mar;19(3):341-6. Epub 2010 Dec 8. PubMed PMID: 21150882; PubMed Central PMCID: PMC3061997.

    Zhang G, Karns R, Narancic NS, Sun G, Cheng H, Missoni S, Durakovic Z, Rudan P, Chakraborty R, Deka R. Common SNPs in FTO gene are associated with obesity related anthropometric traits in an island population from the eastern Adriatic coast of Croatia. PLoS One. 2010 Apr 28;5(4):e10375. PubMed PMID: 20442772;
    PubMed Central PMCID: PMC2860984.

    Deka R, Xu L, Pal P, Toelupe PT, Laumoli TS, Xi H, Zhang G, Weeks DE, McGarvey ST. A tagging SNP in INSIG2 is associated with obesity-related phenotypes among Samoans. BMC Med Genet. 2009 Dec 22;10:143. PubMed PMID: 20028541; PubMed Central PMCID: PMC2804583.

    Nebert DW, Zhang G, Vesell ES. From human genetics and genomics to pharmacogenetics and pharmacogenomics: past lessons, future directions. Drug Metab Rev. 2008;40(2):187-224. Review. PubMed PMID: 18464043; PubMed Central PMCID: PMC2752627.

    Bernstein JA, Zhang G, Jin L, Abbott C, Nebert DW. Olfactory receptor gene polymorphisms and nonallergic vasomotor rhinitis. J Asthma. 2008 May;45(4):287-92. PubMed PMID: 18446592; PubMed Central PMCID: PMC2752410.

    Zhang G, Nebert DW, Chakraborty R, Jin L. Statistical power of association using the extreme discordant phenotype design. Pharmacogenet Genomics. 2006 Jun;16(6):401-13. PubMed PMID: 16708049.

 Alexey Porollo, PhD—Dr. Porollo with CEG Bioinformatic Core directors Mario Medvedovic and Jarek Meller

  • Co-investigator, “Norovirus Capsid: a Novel Drug Target” pending
  •  Co-investigator, “The Role of CD44 in the Hierarchy of Pro-Metastatic Signals in Breast Cancer” pending
  • Participated in course development of applying bioinformatics portals to research, as part of the NIEHS T32 Gene-environment interactions course development.
  • Joined CEG Integrative Health Sciences Core, supporting the Core with his bioinformatics expertise.
  • Awarded CEG Director’s Funds to attend professional meeting.
  • Read about his work in NIEHS eFactor newsletter

Publications:

Syed K, Porollo A, Lam YW, Yadav JS. A fungal P450 (CYP5136A3) capable of oxidizing polycyclic aromatic hydrocarbons and endocrine disrupting alkylphenols: role of Trp(129) and Leu(324). PLoS One. 2011;6(12):e28286. Epub 2011 Dec 2. PubMed PMID: 22164262; PubMed Central PMCID: PMC3229547.

Duran A, Amanchy R, Linares JF, Joshi J, Abu-Baker S, Porollo A, Hansen M, Moscat J, Diaz-Meco MT. p62 is a key regulator of nutrient sensing in the mTORC1 pathway. Mol Cell. 2011 Oct 7;44(1):134-46. PubMed PMID: 21981924; PubMed Central PMCID: PMC3190169.

Giridhar PV, Funk HM, Gallo CA, Porollo A, Mercer CA, Plas DR, Drew AF. Interleukin-6 receptor enhances early colonization of the murine omentum by upregulation of a mannose family receptor, LY75, in ovarian tumor cells. Clin Exp Metastasis. 2011 Dec;28(8):887-97. Epub 2011 Sep 2. PubMed PMID: 21887537.

Biesiada J, Porollo A, Velayutham P, Kouril M, Meller J. Survey of public domain software for docking simulations and virtual screening. Hum Genomics. 2011 Jul;5(5):497-505. Review. PubMed PMID: 21807604.

Swaminathan K, Adamczak R, Porollo A, Meller J. Enhanced prediction of conformational flexibility and phosphorylation in proteins. Adv Exp Med Biol. 2010;680:307-19. PubMed PMID: 20865514.

Porollo A, Meller J. POLYVIEW-MM: web-based platform for animation and analysis of molecular simulations. Nucleic Acids Res. 2010 Jul;38(Web Server issue):W662-6. Epub 2010 May 26. PubMed PMID: 20504857; PubMed Central PMCID: PMC2896192.

Porollo A, Meller J. Versatile annotation and publication quality visualization of protein complexes using POLYVIEW-3D. BMC Bioinformatics. 2007 Aug 29;8:316. PubMed PMID: 17727718; PubMed Central PMCID: PMC1978507.

Cushion MT, Smulian AG, Slaven BE, Sesterhenn T, Arnold J, Staben C, Porollo A, Adamczak R, Meller J. Transcriptome of Pneumocystis carinii during fulminate infection: carbohydrate metabolism and the concept of a compatible parasite. PLoS One. 2007 May 9;2(5):e423. PubMed PMID: 17487271; PubMed Central PMCID: PMC1855432.

Slaven BE, Meller J, Porollo A, Sesterhenn T, Smulian AG, Cushion MT. Draft assembly and annotation of the Pneumocystis carinii genome. J Eukaryot Microbiol. 2006;53 Suppl 1:S89-91. PubMed PMID: 17169080.

Slaven BE, Porollo A, Sesterhenn T, Smulian AG, Cushion MT, Meller J. Large-scale characterization of introns in the Pneumocystis carinii genome. J Eukaryot Microbiol. 2006;53 Suppl 1:S151-3. PubMed PMID: 17169040.

Porollo A, Meller J. Prediction-based fingerprints of protein-protein interactions. Proteins. 2007 Feb 15;66(3):630-45. PubMed PMID: 17152079.

Cao B, Porollo A, Adamczak R, Jarrell M, Meller J. Enhanced recognition of protein transmembrane domains with prediction-based structural profiles. Bioinformatics. 2006 Feb 1;22(3):303-9. Epub 2005 Nov 17. PubMed PMID: 16293670.

Wagner M, Adamczak R, Porollo A, Meller J. Linear regression models for solvent accessibility prediction in proteins. J Comput Biol. 2005 Apr;12(3):355-69. PubMed PMID: 15857247.

Adamczak R, Porollo A, Meller J. Combining prediction of secondary structure and solvent accessibility in proteins. Proteins. 2005 May 15;59(3):467-75. PubMed PMID: 15768403.

Adamczak R, Porollo A, Meller J. Accurate prediction of solvent accessibility using neural networks-based regression. Proteins. 2004 Sep 1;56(4):753-67. PubMed PMID: 15281128.

  • Presentations:
    • Porollo A, Collins MS, Cushion MT (2010) QSAR study of new inhibitors for Pneumocystis carinii oxidosqualene cyclase. 11th International Workshops for Opportunistic Protists, Hilo, HI.
    • Porollo A, Meller J (2010) POLYVIEW: Web-based Platform for Protein Structure Analysis and Visualization. 18th Annual International Conference on Intelligent Systems for Molecular Biology (ISMB 2010), Boston, MA.
    • Phatak M,Cao B, Adamczak R, Porollo A, Wagner M, Meller J (2010) Prediction of Interaction Sites in Membrane Proteins. 5th Annual Ohio Collaborative Conference on Bioinformatics, Columbus, OH.

Yuet-kin “Ricky” Leung—Dr. Ricky Leung

  • R01 submitted (R01CA160512-01) Phosphorylation of estrogen receptor beta in breast cancer.   Pending IRG Review.
  • Publications, including one manuscript in collaboration with a community based participatory research project (1R01ES016531-03) under the direction of CEG member Erin Haynes:
    • Ho SM, Lee MT, Lam HM, Leung YK. Estrogens and prostate cancer: etiology, mediators, prevention, and management. Endocrinol Metab Clin North Am. 2011 Sep;40(3):591-614. Epub 2011 Jul 7. PubMed PMID: 21889723; PubMed Central PMCID:  PMC3167093.
    • Leung YK, Ho SM. Estrogen receptor β: switching to a new partner and escaping from estrogen. Sci Signal. 2011 Apr 12;4(168):pe19. PubMed PMID: 21487104.
    • Ouyang B, Leung YK, Wang V, Chung E, Levin L, Bracken B, Cheng L, Ho SM. α-Methylacyl-CoA racemase spliced variants and their expression in normal and malignant prostate tissues. Urology. 2011 Jan;77(1):249.e1-7. PubMed PMID: 21195844
    • Leung YK, Lam HM, Wu S, Song D, Levin L, Cheng L, Wu CL, Ho SM. Estrogen receptor beta2 and beta5 are associated with poor prognosis in prostate cancer, and promote cancer cell migration and invasion. Endocr Relat Cancer. 2010 Jun 25;17(3):675-89. Print 2010. PubMed PMID: 20501637; PubMed Central PMCID: PMC2891483.
    •  Zhang X, Wu M, Xiao H, Lee MT, Levin L, Leung YK, Ho SM. Methylation of a single intronic CpG mediates expression silencing of the PMP24 gene in prostate cancer. Prostate. 2010 May 15;70(7):765-76. PubMed PMID: 20054818; PubMed Central PMCID: PMC2857536.
    • Haynes EN, Heckel P, Ryan P, Roda S, Leung YK, Sebastian K, Succop P. Environmental manganese exposure in residents living near a ferromanganese refinery in Southeast Ohio: A pilot study. Neurotoxicology. 2010 Sept;31(5):468-74 PubMed PMID: 19879291

 

Opeolu Adeoye, MD—Dr. Opeolu Adeoye

·         2010 CEG Pilot Project Grant “Epigenetic markers of stroke type”  funded.  This pilot project provided preliminary data K23 application “Hemostasis in intracerebral hemorrhage” K23NS075143-01  pending

  • Junior T1 Pilot Award from the CCTST.  Metalloproteins in Stoke Diagnosis.  Co-investigator CEG member Joseph Caruso.

·         Subcontract $33,409 for Clear IVH III-Clot Lysis: Evaluating Accelerated Resolution of Intraventricular Hemorrhage Phase III, U01NS06285, Daniel Hanley, PI.  Clot Lysis: Evaluating accelerated resolution of IVH Phase II (CLEAR III).

  • Subcontract to examine patient-participants on seven other awards.

·         Featured case report:  Neurocritical Care

·         Publications since NGBI award:

·         Kleindorfer D, Lindsell CJ, Alwell K, Woo D, Flaherty ML, Eilerman J, Khatri P, Adeoye O, Ferioli S, Kissela BM. Ischemic Stroke Survivors' Opinion Regarding  Research Utilizing Exception from Informed Consent. Cerebrovasc Dis. 2011 Sep 15;32(4):321-326. [Epub ahead of print] PubMed PMID: 21921594.

·         Broderick JP, Bonomo JB, Kissela BM, Khoury JC, Moomaw CJ, Alwell K, Woo D, Flaherty ML, Khatri P, Adeoye O, Ferioli S, Kleindorfer DO. Withdrawal of antithrombotic agents and its impact on ischemic stroke occurrence. Stroke. 2011  Sep;42(9):2509-14. Epub 2011 Jun 30. PubMed PMID: 21719769; PubMed Central PMCID: PMC3166233.

·         Butler SM, Abrassart JM, Hubbell MC, Adeoye O, Semotiuk A, Williams JM, Mata-Greenwood E, Khorram O, Pearce WJ. Contributions of VEGF to age-dependent transmural gradients in contractile protein expression in ovine carotid arteries. Am J Physiol Cell Physiol. 2011 Sep;301(3):C653-66. Epub 2011 Jun 8. PubMed PMID 21653901; PubMed Central PMCID: PMC3174571.

·         Adeoye O, Hornung R, Khatri P, Kleindorfer D. Recombinant tissue-type plasminogen activator use for ischemic stroke in the United States: a doubling of treatment rates over the course of 5 years. Stroke. 2011 Jul;42(7):1952-5. Epub 2011 Jun 2. PubMed PMID: 21636813.

·         Mackey J, Kleindorfer D, Sucharew H, Moomaw CJ, Kissela BM, Alwell K, Flaherty ML, Woo D, Khatri P, Adeoye O, Ferioli S, Khoury JC, Hornung R, Broderick JP. Population-based study of wake-up strokes. Neurology. 2011 May 10;76(19):1662-7.  PubMed PMID: 21555734; PubMed Central PMCID: PMC3100086.

·         Adeoye O, Pancioli A, Khoury J, Moomaw CJ, Schmit P, Ewing I, Alwell K, Flaherty ML, Woo D, Ferioli S, Khatri P, Broderick JP, Kissela BM, Kleindorfer D. Efficiency of Enrollment in a Successful Phase II Acute Stroke Clinical Trial. J  Stroke Cerebrovasc Dis. 2011 Apr 2. [Epub ahead of print] PubMed PMID: 21459614;  PubMed Central PMCID: PMC3130072.

·         Adeoye O. Control of elevated blood pressure in acute intracerebral hemorrhage. F1000 Med Rep. 2010 Oct 21;2:75. PubMed PMID: 21173835; PubMed Central PMCID: PMC2981178.

·         Cairns CB, Maier RV, Adeoye O, Baptiste D, Barsan WG, Blackbourne L, Burd R, Carpenter C, Chang D, Cioffi W, Cornwell E, Dean JM, Dyer C, Jaffe D, Manley G, Meurer WJ, Neumar R, Silbergleit R, Stevens M, Wang M, Weiner D, Wright D; Roundtable External Participants and Roundtable Steering Committee and Federal Participants. NIH Roundtable on Emergency Trauma Research. Ann Emerg Med. 2010 Nov;56(5):538-50. PubMed PMID: 21036294.

·         Jauch EC, Cucchiara B, Adeoye O, Meurer W, Brice J, Chan YY, Gentile N, Hazinski MF. Part 11: adult stroke: 2010 American Heart Association Guidelines for Cardiopulmonary Resuscitation and Emergency Cardiovascular Care. Circulation. 2010 Nov 2;122(18 Suppl 3):S818-28. Review. PubMed PMID: 20956227.

·         Adeoye O, Broderick JP. Advances in the management of intracerebral hemorrhage. Nat Rev Neurol. 2010 Nov;6(11):593-601. Epub 2010 Sep 28. Review. PubMed PMID: 20877400.

·         Adeoye O, Haverbusch M, Woo D, Sekar P, Moomaw CJ, Kleindorfer D, Stettler B, Kissela BM, Broderick JP, Flaherty ML. Is ED disposition associated with intracerebral hemorrhage mortality? Am J Emerg Med. 2011 May;29(4):391-5. Epub 2010 Mar 25. PubMed PMID: 20825807; PubMed Central PMCID: PMC3005610.

·         Adeoye O, Heitsch L, Moomaw CJ, Alwell K, Khoury J, Woo D, Flaherty ML, Ferioli S, Khatri P, Broderick JP, Kissela BM, Kleindorfer D. How much would performing diffusion-weighted imaging for all transient ischemic attacks increase MRI utilization? Stroke. 2010 Oct;41(10):2218-22. Epub 2010 Aug 26. PubMed PMID:20798366; PubMed Central PMCID: PMC2952926.

·         Adeoye O, Hornung R, Khatri P, Ringer A, Kleindorfer D. The rate of hemicraniectomy for acute ischemic stroke is increasing in the United States. J Stroke Cerebrovasc Dis. 2011 May-Jun;20(3):251-4. Epub 2010 Jul 10. PubMed PMID: 20621514.

·         Kleindorfer D, Lindsell CJ, Moomaw CJ, Alwell K, Woo D, Flaherty ML, Adeoye O, Zakaria T, Broderick JP, Kissela BM. Which stroke symptoms prompt a 911 call? A population-based study. Am J Emerg Med. 2010 Jun;28(5):607-12. Epub 2010 Jan 28.  PubMed PMID: 20579558.

·         Kleindorfer DO, Khoury J, Moomaw CJ, Alwell K, Woo D, Flaherty ML, Khatri P, Adeoye O, Ferioli S, Broderick JP, Kissela BM. Stroke incidence is decreasing in  whites but not in blacks: a population-based estimate of temporal trends in stroke incidence from the Greater Cincinnati/Northern Kentucky Stroke Study. Stroke. 2010 Jul;41(7):1326-31. Epub 2010 May 20. PubMed PMID: 20489177; PubMed Central PMCID: PMC2904073.

·         Adeoye O, Ringer A, Hornung R, Khatri P, Zuccarello M, Kleindorfer D. Trends in surgical management and mortality of intracerebral hemorrhage in the United States before and after the STICH trial. Neurocrit Care. 2010 Aug;13(1):82-6. PubMed PMID: 20383612.

·         Khan SH, Adeoye O, Abruzzo TA, Shutter LA, Ringer AJ. Intracranial dural sinus thrombosis: novel use of a mechanical thrombectomy catheter and review of management strategies. Clin Med Res. 2009 Dec;7(4):157-65. Review. PubMed PMID: 20048139; PubMed Central PMCID: PMC2801689.

·         Kleindorfer D, Xu Y, Moomaw CJ, Khatri P, Adeoye O, Hornung R. US geographic distribution of rt-PA utilization by hospital for acute ischemic stroke. Stroke.  2009 Nov;40(11):3580-4. Epub 2009 Oct 1. PubMed PMID: 19797701.

·         Worster A, Keim SM, Carpenter CR, Adeoye O; Best Evidence in Emergency Medicine (BEEM) Group. Does early intensive lowering of blood pressure reduce hematoma volume and improve clinical outcome after acute cerebral hemorrhage? J Emerg Med. 2009 Nov;37(4):433-8. Epub 2009 Jul 9. Review. PubMed PMID: 19545967.

·         Adeoye O, Lindsell C, Broderick J, Alwell K, Jauch E, Moomaw CJ, Flaherty ML,  Pancioli A, Kissela B, Kleindorfer D. Emergency medical services use by stroke patients: a population-based study. Am J Emerg Med. 2009 Feb;27(2):141-5. PubMed  PMID: 19371519; PubMed Central PMCID: PMC2686130.

·         Flaherty ML, Adeoye O, Sekar P, Haverbusch M, Moomaw CJ, Tao H, Broderick JP,  Woo D. The challenge of designing a treatment trial for warfarin-associated intracerebral hemorrhage. Stroke. 2009 May;40(5):1738-42. Epub 2009 Mar 12. PubMed PMID: 19286588; PubMed Central PMCID: PMC2674527.

·         Adeoye O, Woo D, Haverbusch M, Sekar P, Moomaw CJ, Broderick J, Flaherty ML.  Surgical management and case-fatality rates of intracerebral hemorrhage in 1988 and 2005. Neurosurgery. 2008 Dec;63(6):1113-7; discussion 1117-8. PubMed PMID: 19057323; PubMed Central PMCID: PMC2717618.

·         Flaherty ML, Tao H, Haverbusch M, Sekar P, Kleindorfer D, Kissela B, Khatri P, Stettler B, Adeoye O, Moomaw CJ, Broderick JP, Woo D. Warfarin use leads to larger intracerebral hematomas. Neurology. 2008 Sep 30;71(14):1084-9. PubMed PMID: 18824672; PubMed Central PMCID: PMC2668872.

·         Adeoye O, Woo D, Haverbusch M, Tao H, Sekar P, Moomaw CJ, Shutter L, Kleindorfer D, Kissela B, Broderick J, Flaherty ML. Eligibility for the surgical  trial in intracerebral hemorrhage II study in a population-based cohort. Neurocrit Care. 2008;9(2):237-41. PubMed PMID: 18183500; PubMed Central PMCID: PMC2672191.

 

2009

Guo-Chang Fan, PhD—In addition to the CEG NGBI award, Dr. Fan has received support to use CEG core facilities (flow cytometry in ITSC and Bioinformatics).  He is an author on ten publications from 2009-11, including a Circulation paper with support from the CEG Bioinformatics Core.

Grants

  • After his CEG NGBI award his revision of Physiological and Pathophysiological Roles of Hsp20 in the Heart (HL087861) was renewed. 
  • He has submitted Role of the MicroRNA-144/451 Cluster in Myocardial Ischemic Damage and Ischemic Tolerance (4/12-3/17 $2,226,444  Pending) using data collected with CEG support.

Publications:

·         Huang W, Wang T, Zhang D, Zhao T, Dai B, Ashraf A, Wang X, Xu M, Millard RW, Fan GC, Ashraf M, Yu XY, Wang Y. Mesenchymal Stem Cells Overexpressing CXCR4 Attenuate Remodeling of Postmyocardial Infarction by Releasing Matrix Metalloproteinase-9. Stem Cells Dev. 2011 Jul 22. [Epub ahead of print] PubMed PMID: 21671800.

·         Qian J, Vafiadaki E, Florea SM, Singh VP, Song W, Lam CK, Wang Y, Yuan Q, Pritchard TJ, Cai W, Haghighi K, Rodriguez P, Wang HS, Sanoudou D, Fan GC, Kranias EG. Small heat shock protein 20 interacts with protein phosphatase-1 and  enhances sarcoplasmic reticulum calcium cycling. Circ Res. 2011 Jun 10;108(12):1429-38. Epub 2011 Apr 14. PubMed PMID: 21493896; PubMed Central PMCID: PMC3125589.

·         Zuo S, Jones WK, Li H, He Z, Pasha Z, Yang Y, Wang Y, Fan GC, Ashraf M, Xu M.  Paracrine Effect of Wnt11-Overexpressing Mesenchymal Stem Cells on Ischemic Injury. Stem Cells Dev. 2011 Jun 1. [Epub ahead of print] PubMed PMID: 21463175;  PubMed Central PMCID: PMC3156900.

·         Wang X, Zhang X, Ren XP, Chen J, Liu H, Yang J, Medvedovic M, Hu Z, Fan GC. MicroRNA-494 targeting both proapoptotic and antiapoptotic proteins protects against ischemia/reperfusion-induced cardiac injury. Circulation. 2010 Sep 28;122(13):1308-18. Epub 2010 Sep 13. PubMed PMID: 20837890; PubMed Central PMCID: PMC2956502.

·         Zhang X, Wang X, Zhu H, Zhu C, Wang Y, Pu WT, Jegga AG, Fan GC. Synergistic effects of the GATA-4-mediated miR-144/451 cluster in protection against simulated ischemia/reperfusion-induced cardiomyocyte death. J Mol Cell Cardiol. 2010 Nov;49(5):841-50. Epub 2010 Aug 11. PubMed PMID: 20708014; PubMed Central PMCID: PMC2949485.

·         Chen G, Zhou X, Florea S, Qian J, Cai W, Zhang Z, Fan GC, Lorenz J, Hajjar RJ, Kranias EG. Expression of active protein phosphatase 1 inhibitor-1 attenuates chronic beta-agonist-induced cardiac apoptosis. Basic Res Cardiol. 2010 Sep;105(5):573-81. Epub 2010 May 30. PubMed PMID: 20512582.

·         Huang W, Zhang D, Millard RW, Wang T, Zhao T, Fan GC, Ashraf A, Xu M, Ashraf M, Wang Y. Gene manipulated peritoneal cell patch repairs infarcted myocardium. J Mol Cell Cardiol. 2010 Apr;48(4):702-12. Epub 2009 Nov 11. PubMed PMID: 19913551; PubMed Central PMCID: PMC2905838.

·         Qian J, Ren X, Wang X, Zhang P, Jones WK, Molkentin JD, Fan GC, Kranias EG.  Blockade of Hsp20 phosphorylation exacerbates cardiac ischemia/reperfusion injury by suppressed autophagy and increased cell death. Circ Res. 2009 Dec 4;105(12):1223-31. Epub 2009 Oct 22. PubMed PMID: 19850943; PubMed Central PMCID: PMC2799045.

·         Wang X, Zhao T, Huang W, Wang T, Qian J, Xu M, Kranias EG, Wang Y, Fan GC. Hsp20-engineered mesenchymal stem cells are resistant to oxidative stress via enhanced activation of Akt and increased secretion of growth factors. Stem Cells. 2009 Dec;27(12):3021-31. PubMed PMID: 19816949; PubMed Central PMCID: PMC2806498.

·         Jones WK, Fan GC, Liao S, Zhang JM, Wang Y, Weintraub NL, Kranias EG, Schultz  JE, Lorenz J, Ren X. Peripheral nociception associated with surgical incision elicits remote nonischemic cardioprotection via neurogenic activation of protein  kinase C signaling. Circulation. 2009 Sep 15;120(11 Suppl):S1-9. PubMed PMID: 19752352; PubMed Central PMCID: PMC2845316.

 

John Reichard, PhD, PharmD—

·         2009 CEG Pilot Project Grant $22,500 Regulation of histone methylation by arsenite.

·         2008/2009 CEG Director’s Discretionary Funds $30,000 each year; $60,000 total

·         Invited speaker to present at 2010 Arsenic workshop held as a satellite to the NIEHS Annual Centers meeting in Louisville, Kentucky.

·         Submitted R01ES018844 “Epigenetic regulation of gene activity by arsenic in human keratinocytes” Council review completed. 

·         In 2011, started collaboration with CEG partner TERA (Toxicology Excellence for Risk Assessment)

·         Co-Investigator with CEG member Medvedovic (Bioinformatics Core leader) on “Integrative statistical methods and tools for analysis of perturbation signatures” U01HL111638-01 9/11-6/30 $772,670 Awarded

Publications:

  • States JC, Barchowsky A, Cartwright IL, Reichard JF, Futscher BW, Lantz RC. Arsenic Toxicology: Translating between Experimental Models and Human Pathology.  Environ Health Perspect. 2011 Jun 17. [Epub ahead of print] PubMed PMID: 21684831.
  • Reichard JF, Puga A. Effects of arsenic exposure on DNA methylation and epigenetic gene regulation. Epigenomics. 2010 Feb 1;2(1):87-104. PubMed PMID: 20514360; PubMed Central PMCID: PMC2877392.
  • Sartor MA, Schnekenburger M, Marlowe JL, Reichard JF, Wang Y, Fan Y, Ma C, Karyala S, Halbleib D, Liu X, Medvedovic M, Puga A. Genomewide analysis of aryl hydrocarbon receptor binding targets reveals an extensive array of gene clusters  that control morphogenetic and developmental programs. Environ Health Perspect. 2009 Jul;117(7):1139-46. Epub 2009 Mar 24. PubMed PMID: 19654925; PubMed Central  PMCID: PMC2717142.

 

2010

Iris Gutmark-Little, MD is working with CEG members Ho, Belcher and Leung to investigate environmental disruptors of linear growth, to identify environmental agents that potentially disrupt or alter epiphyseal development and maturation. 

2010-2011

Ana Luisa Kadekaro, PhDDr. Kadekaro faces the challenge of being mentored by a top-producing CEG member, Dr. Zalfa Abdel-Malek.  She requested CEG support for a short (couple of weeks) sabbatical to another lab where she could learn techniques novel to the lab, and help to establish herself as an independent investigator.  She has received an NIEHS R21, a 2011 CEG Pilot Project.  As part of her training to be a mentor, CEG supplied DDF money for a SURF (Summer Undergraduate Research Fellow) student for summers 2010 and 2011.  CEG COEC also supported her work as organizer of the Molecular and Cancer Cell Biology section of the 2011 Howard Hughes Excellence in Science Education and Learning Program (ExCEL).  CEG provided money for hands-on activities (running gels, etc), and CEG members provided tours of CEG Facility Cores.  Twenty high school students enrolled in this program.

Dr. Kadekaro reported on the findings of her research at the NIEHS P30 Centers meeting in Louisville in 2010, and is currently preparing her first R01 application--to NIEHS.

·         R21ES016900 “Defining a New Role for Alpha-MSH: Antioxidant in UVB-Irradiated Melanocytes” funded 7/7/10-6/30/12 $431,750.

·         2011 CEG Pilot Grant

·         Dermatology Foundation award $20,000 SRS 006872 “Increase in p53 Expression as a Strategy for Melanoma Prevention” 7/1/2010-6/30/2011

·         Publications:

o    Kadekaro AL, Leachman S, Kavanagh RJ, Swope V, Cassidy P, Supp D, Sartor M, Schwemberger S, Babcock G, Wakamatsu K, Ito S, Koshoffer A, Boissy RE, Manga P, Sturm RA, Abdel-Malek ZA. Melanocortin 1 receptor genotype: an important determinant of the damage response of melanocytes to ultraviolet radiation. FASEB J. 2010 Oct;24(10):3850-60.  PMID: 20519635.

o   Abdel-Malek ZA, Kadekaro AL, Swope VB. Stepping up melanocytes to the challenge of UV exposure. Pigment Cell Melanoma Res. 2010 Apr;23(2):171-86. Review. PMID: 20128873.

 

2011

Yuhang Zhang, PhD, is a tenure track assistant professor in Pharmacy with a secondary appointment in Dermatology.  He wants to elucidate the genetic and epigenetic programs implicated in skin melanocytes homeostasis under the complex relationships between genetic composition and environmental exposure.  Dr. Zhang received a 2011 CEG Pilot Project Grant for

Ian Lewkowich, PhD, is a research instructor at CCHMC.  He has proposed to identify novel quantitative trait loci (QTLs) driving pulmonary dendritic cell activation following exposure to environmental allergens, using advanced recombinant inbred BxD mice.

New Investigator Scholar Program  The second pathway funded directly by the Career Development Core is the New Investigator Scholar (NIS) award, designed to assist clinicians and health practitioners pursuing post-graduate studies, post-doctoral fellows and graduate students learn environmental health science concepts, and bring these concepts into translational research practice.  Support is provided to students for dissertation research expenses, career development, travel to professional meetings, and other activities.  Awards are from usually between $2500 - $5,000. 

The CEG has supported nineteen NISs from 2008 to present.  Benchmarks for these students, usually clinical fellows working on an additional degree or certification in clinical research, include presentations, passing qualifiers or dissertations, and participating in career development activities.  Some benchmarks are listed below.

Stephen Adjei, MD, MPH, Clinical Fellow, Division of Pulmonary Medicine, Department of Pulmonary Medicine 

  • Served as a mentor for urban African American high school students interested in health sciences careers
  • Worked with CEG members LeMasters, Lockey, and Ryan to prepare R21ES017939-01 The Libby Community Childhood Health Investigation and Exposure Follow-Up Study. Funded 9/17/09-6/30/13  $1,094,158 total costs.
    • Ryan PH, Dihle M, Griffin S, Partridge C, Hilbert TJ, Taylor R, Adjei S, Lockey JE. Erionite in road gravel associated with interstitial and pleural changes--an occupational hazard in western United States. J Occup Environ Med. 2011 Aug;53(8):892-8. PubMed PMID: 21775901.
    • Yousuf MA, Adjei S, Kinder B. A 58-year-old woman with ST-segment elevation, seizures, and altered mental status in the setting of opiate withdrawal. Chest. 2009 Apr;135(4):1098-101. PubMed PMID: 19349407.

Tolly Epstein, MD, Division of Allergy and Immunology, Internal Medicine, College of Medicine was promoted from Fellow to Assistant Professor this year.  She successfully submitted a KL2 award through the Center for Clinical and Translational Science Training, our local CTSA.  Epstein is on her way to making an impact as a clinician-scientist. 

  • Awarded 2010 CEG pilot project “Air pollutants, anti-oxidant genes and asthma severity in older adults” with CEG mentors Bernstein and Deka.
  • Awarded 2011 CEG pilot project
  • Poster, 2008 EHS Showcase
    • Bernstein DI, Epstein T. Systemic reactions to subcutaneous allergen immunotherapy. Immunol Allergy Clin North Am. 2011 May;31(2):241-9, viii-ix. Review. PubMed PMID: 21530817.
    • Epstein TG, Liss GM, Murphy-Berendts K, Bernstein DI. Immediate and delayed-onset systemic reactions after subcutaneous immunotherapy injections: ACAAI/AAAAI surveillance study of subcutaneous immunotherapy-year 2. Ann Allergy  Asthma Immunol. 2011 Nov;107(5):426-431.e1. Epub 2011 Jun 17. PubMed PMID: 22018614.
    • Epstein TG, Bernstein DI, Levin L, Khurana Hershey GK, Ryan PH, Reponen T, Villareal M, Lockey JE, Lemasters GK. Opposing effects of cat and dog ownership and allergic sensitization on eczema in an atopic birth cohort. J Pediatr. 2011 Feb;158(2):265-71.e1-5. PubMed PMID: 20884006.
    • Bernstein DI, Epstein T, Murphy-Berendts K, Liss GM. Surveillance of systemic  reactions to subcutaneous immunotherapy injections: year 1 outcomes of the ACAAI  and AAAAI collaborative study. Ann Allergy Asthma Immunol. 2010 Jun;104(6):530-5 PubMed PMID: 20568387.
    • Biagini Myers JM, Wang N, LeMasters GK, Bernstein DI, Epstein TG, Lindsey MA,  Ericksen MB, Chakraborty R, Ryan PH, Villareal MS, Burkle JW, Lockey JE, Reponen  T, Khurana Hershey GK. Genetic and environmental risk factors for childhood eczema development and allergic sensitization in the CCAAPS cohort. J Invest Dermatol. 2010 Feb;130(2):430-7. Epub 2009 Sep 17. PubMed PMID: 19759553; PubMed  Central PMCID: PMC2807898.
    • Epstein TG, Bernstein JA. Current and emerging management options for hereditary angioedema in the US. Drugs. 2008;68(18):2561-73. doi: 10.2165/0003495-200868180-00003. Review. PubMed PMID: 19093699.

David Hooper, MD, Clinical Fellow, Division of Nephrology and Hypertension, Cincinnati Children’s Hospital and Medical Center   (received second NIS award in 2010)A photo of Dr. David Hooper.

  • Center for Education and Research in Therapeutics award for “Genotype as a predictor of mycophenolate mofetil related complications in pediatric kidney transplant patients” 3/09-9/09 $10,000. 
  • Clinical and Translational Science Awards (CTSA) KL2 Research Scholars
    Title: A reliable system for blood pressure control
    Primary Mentor: Peter Margolis, MD PhD
    Funding: $200,000, Period: 04/2012 – 03/2014
  • Place Outcomes Research Award, Cincinnati Children’s Hospital
    Title: A reliable blood pressure management system.
    Principal Investigator: David K. Hooper, MD
    Primary Mentor: Peter Margolis, MD PhD
    Funding: $70,000
    Period: 07/2012 – 06/2014
     
  • Presenter, “Intravenous Nicardipine:A ‘Diagnostic Test’ for CYP3A5 Polymorphisms in Tacrolimus- Treated Patients”  January 28, 2010.
  • Poster, 2008 EHS Showcase

    Hooper DK, Carle AC, Schuchter J, Goebel J. Interaction between tacrolimus and intravenous nicardipine in the treatment of post-kidney transplant hypertension at pediatric hospitals. Pediatr Transplant. 2011 Feb;15(1):88-95. doi: 10.1111/j.1399-3046.2010.01417.x. Epub 2010 Dec 15. PubMed PMID: 21159108.

    Kaplan HC, Brady PW, Dritz MC, Hooper DK, Linam WM, Froehle CM, Margolis P. The influence of context on quality improvement success in health care: a systematic review of the literature. Milbank Q. 2010 Dec;88(4):500-59. doi: 10.1111/j.1468-0009.2010.00611.x. Review. PubMed PMID: 21166868.

Saldaña SN, Hooper DK, Froehlich TE, Campbell KM, Prows CA, Sadhasivam s, Nick TG, Seid M, Vinks AA, Glauser TA. Characteristics of Successful Recruitment in Prospective Pediatric Pharmacogenetic Studies. Clinical Therapeutics. 2011 Dec; 33(12):2072-81.

Hooper DK, Fukuda T, Logan B, Gardiner R, Roy-Chaudhury A, Kirby C, Vinks A, Goebel J. Risk of Tacrolimus Toxicity in CYP3A5 Non-Expressors Treated with Intravenous Nicardipine After Kidney Transplantation. Transplantation. 2012 Apr;93(8):806-812.

 

 Kelly Metz, MD, Clinical Fellow, Division of Allergy and Immunology, Cincinnati Children’s Hospital and Medical Center

  • Awarded 2009 CEG Pilot Project award “The impact of second hand smoke (SHS) on the clinical and molecular phenotypes of children hospitalized with hospitalized asthma exacerbation and the response to the standard therapy” with her mentor, Hershey.
  • Poster, 2008 EHS Showcase

 Nicholas Newman, DO, FAAP, Clinical Fellow, Molecular Epidemiology in Children’s Environmental Health, Department of Environmental Health.

  • Invited moderator of EHS Showcase Sept 2009
  • Traveled to ISEE for environmental epidemiology conference with IHSFC director Susan Pinney. 
  • Invited moderator for IHS Core Forth and Back conference, January 29, 2010
  • Director Cincinnati Children’s Hospital and Medical Center Pediatric Environmental Health Specialty Unit, 2010.
  • Will finish MS degree in 2011 and residency program in Occupational and Environmental Medicine.
  • Joined CEG COEC as pediatric education specialist, producing one module on the role of the environment in children’s health http://www.eh.uc.edu/CARES/learn/physicians.html

 Christopher Codispoti, MD, Clinical Fellow, Division of Allergy and Immunology, Cincinnati Children’s Hospital and Medical Center

  • PhD, 2011, as fellow in Molecular Epidemiology and Children’s Environmental Health NIEHS T32 program.
  • Codispoti CD, Levin L, LeMasters GK, Ryan P, Reponen T, Villareal M, Burkle J, Stanforth S, Lockey JE, Khurana Hershey GK, Bernstein DI. Breast-feeding, aeroallergen sensitization, and environmental exposures during infancy are determinants of childhood allergic rhinitis. J Allergy Clin Immunol. 2010 May;125(5):1054-1060.e1. Epub 2010 Apr 14. PubMed PMID: 20392478.  This article was selected as one of the 8 best articles in 2010 on allergic rhinitis.
  • Raja AS, Lindsell CJ, Bernstein JA, Codispoti CD, Moellman JJ. The use of penicillin skin testing to assess the prevalence of penicillin allergy in an emergency department setting. Ann Emerg Med. 2009 Jul;54(1):72-7. Epub 2009 Feb 13. PubMed PMID: 19217696.

2010 NIS awardees. 

Brian Wortham, PhD is a postdoctoral fellow in Michael Borchers’ lab.  He is supported by an NIEHS T32 Gene-environment interactions training program. 

  • Eppert BL, Motz GT, Wortham BW, Flury JL, Borchers MT. CCR7 deficiency leads to leukocyte activation and increased clearance in response to pulmonary Pseudomonas aeruginosa infection. Infect Immun. 2010 May;78(5):2099-107. Epub 2010 Feb 22. PubMed PMID: 20176793; PubMed Central PMCID: PMC2863523.
  • Motz GT, Eppert BL, Wortham BW, Amos-Kroohs RM, Flury JL, Wesselkamper SC, Borchers MT. Chronic cigarette smoke exposure primes NK cell activation in a mouse model of chronic obstructive pulmonary disease. J Immunol. 2010 Apr 15;184(8):4460-9. Epub 2010 Mar 12. PubMed PMID: 20228194.

Haejin Kim, MD is working on a pilot study to measure cytokine and chemokine profiles in nasal supernatants obtained from subjects in year 7 of the Cincinnati Allergy Air Pollution Study (CCAAPS).  She is supported by NIEHS T32 Molecular Epidemiology in Children’s Environmental Health, and her mentor is CEG member David Bernstein. 

2010-2011 NIS awardees

Mansi Krishan is a graduate student in Toxicology, studying under CEG member Mary Beth Genter. She is studying the fate and transport of materials through intra-nasal exposure.  CEG funds supported animal and supply costs for her dissertation research. Krishan was awarded a University Research Council Graduate Student Research Fellowship—summer stipend support.

  • Kendig EL, Chen Y, Krishan M, Johansson E, Schneider SN, Genter MB, Nebert DW, Shertzer HG. Lipid metabolism and body composition in Gclm(-/-) mice. Toxicol Appl Pharmacol. 2011 Sep 24. [Epub ahead of print] PubMed PMID: 21967773.
  • Shertzer HG, Woods SE, Krishan M, Genter MB, Pearson KJ. Dietary whey protein lowers the risk for metabolic disease in mice fed a high-fat diet. J Nutr. 2011Apr 1;141(4):582-7. Epub 2011 Feb 10. PubMed PMID: 21310864; PubMed Central PMCID: PMC3056576.
  • Genter MB, Krishan M, Augustine LM, Cherrington NJ. Drug transporter expression and localization in rat nasal respiratory and olfactory mucosa and olfactory bulb. Drug Metab Dispos. 2010 Oct;38(10):1644-7. Epub 2010 Jul 21. PubMed PMID: 20660103; PubMed Central PMCID: PMC2957163.

Marina Galvez-Peralta, PhD is also a postdoctoral fellow supported by the NIEHS T32 Gene-environment interactions training program, in Daniel Nebert’s lab.  She is working on projects with CEG members Susan Pinney, Susan Kasper, and Tracy Glauser.  Her 2010 award paid to study Apc (+/-) and Foxn1 (-/-) mice and their responses to benzo[a]pyrene.  CEG support in 2011 will enable her to perform histology and electron microscopy on mice to study the role of zip8 transporter on cadmium toxicity. 

Nebert DW, Gálvez-Peralta M, Shi Z, Dragin N. Inbreeding and epigenetics: beneficial as well as deleterious effects. Nat Rev Genet. 2010 Sep;11(9):662. Epub 2010 Jul 27. PubMed PMID: 20661256.

·         Shi Z, Dragin N, Gálvez-Peralta M, Jorge-Nebert LF, Miller ML, Wang B, Nebert DW. Organ-specific roles of CYP1A1 during detoxication of dietary benzo[a]pyrene. Mol Pharmacol. 2010 Jul;78(1):46-57. Epub 2010 Apr 6. PubMed PMID: 20371670; PubMed Central PMCID: PMC2912055.

2011 NIS awardees

Leah Kottyan is a postdoctoral fellow in the newly established Center for Autoimmune Genomics and Etiology (CAGE), under the direction of John Harley at the CCHMC.  The CEG is funding her work to identify increased lupus incidence and somatic mutations in the Fernald Community Cohort, which is managed by the CEG IHSFC. 

Stacey Burgess is a PhD student in the Immunology program at CCHMC, under the mentorship of CEG member Marsha Wills-Karp.  She is studying the role of intestinal microbiota in atopy and asthma, which includes the role that genetic variability plays in microbiota composition.  CEG support will cover animal and lab supplies for this project.

Stephanie R. Sisley is a Clinical Fellow in Pediatric Endocrinology.  She is investigating the role of vitamin D deficiency in the development of obesity and diabetes with CEG member Randy Seeley, which is a new direction for this lab.  CEG will provide funds for equipment and animal studies.

Barbara Kowalcyk is a PhD student in Epidemiology and Biostatistics, and an NIEHS T32 fellow in the Molecular Epidemiology of Children’s Environmental Health (MECEH) program.  She is a nationally recognized foodborne illness prevention advocate, and is supported by the CEG to attend the International Association for Food Protection annual meeting.  She organized and served as a panel member on Long-term health outcomes (LTHO) of foodborne disease: Assessing information needs and methods to capture patient data. 

Stephane Lajoie is a postdoctoral fellow in CEG member Marsha Wills-Karp’s lab. He wants to understand how asthma severity is driven in genetically susceptible mice by epigenetic changes that seem to regulate TH17 cells and the cytokines they produce.  CEG funds will provide him with mice and research supplies for this project.

LaSharon D. Mosley is a postdoctoral fellow on the Molecular Epidemiology and Children’s Environmental Health NIEHS T32.  She is investigating the role of IGF-1 in triple-negative breast cancer development.  Her CEG supported lab supplies and procurement of human tissue and cell lines.  She has been offered a teaching position in the Biology Department at the University of Cincinnati.

Kpandja Djawe, PhD, has graduated from our department, under the mentorship of Dr. Peter Walzer at the Cincinnati Veteran’s Administration Hospital.  He is studying how climatic factors and ambient air pollutants affect PdP hospital admissions and the development of antibody responses against Pneumocystis jirovecii infection in HIV-infected patients.  CEG supported his travel to the Society for Epidemiologic Research annual meeting in Montreal. 

Eileen Alexander is a PhD student in Epidemiology and Biostatistics, where she is currently investigating the recurrence risk of familial eosinophilic esophagitis, by sex and relation.  Her CEG award was for data analysis equipment and travel to a professional meeting.  Eileen has received a 2012 CEG Pilot Project grant to study the role of GxE on eosinophilic esophagitis in twins.

 

Research supported by the Center for Environmental Genetics should cite NIEHS P30-ES006096


 

 

 

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